Press Release Summary
Broad Institute of MIT and Harvard Innovates Living Cell Transcriptome Measurement
Date: September 1
Researchers at the Broad Institute and MIT have developed a groundbreaking method called “cellular self-reporting” that allows living cells to share their RNA transcriptomes without being destroyed. This innovation enables scientists to analyze gene activity over time without providing just a single snapshot.
Key Highlights:
- Traditional methods of measuring a cell’s transcriptome typically require killing the cell, yielding only a one-time snapshot of gene activity.
- The new technique uses engineered virus-like particles that living cells package RNA into and secrete into their surrounding medium.
- Researchers can repeatedly sample this medium for RNA analysis, allowing them to observe changes in cellular behavior associated with aging or drug responses.
Statements from Researchers:
- Paul Blainey, senior author, emphasized the importance of developing practical tools that transform scientific capabilities, noting the method’s journey from concept to realization as a form of innovative research impact.
- Co-first author Mohamad Najia highlighted the broad accessibility of this molecular solution for labs, which could enable deeper insights into dynamic biological questions.
Research Applications:
- Successfully tested in a wide range of cell types, including human, cancer, stem, and primary cells.
- Demonstrated efficacy in complex systems like tissue spheroids and organ-on-a-chip models, enabling studies of gene expression without disturbing three-dimensional structures.
The Broad Institute team continues to explore additional applications and aims to adapt the technology for single-cell studies, inviting researchers to leverage this innovative method for future investigations.
