In our latest evaluation, Mamavation has independently tested the top-rated probiotic, Seed DS-01 Daily Synbiotic, in an EPA-certified lab for potential contaminants, including heavy metals, PFAS “forever chemicals,” and pesticides. We have exciting news to share based on the results! Mamavation has consistently provided thorough investigations into the safest women’s multivitamins, children’s multivitamins, and safest probiotics. Join us as we unveil the results of our ongoing testing of Seed DS-01.
Disclosure: Mamavation funded the laboratory testing to check for possible contamination in this product. The affiliate funds generated from Seed purchases will be used to reimburse ourselves for this testing. This post contains affiliate links, and Mamavation earns a small percentage of sales. We appreciate your support!

Mamavation Sent Seed to the Laboratory to Test for Contaminants
Since 2018, Mamavation has been examining and testing Seed DS-01. Although we had limited resources to conduct an extensive analysis of various probiotics this year, we ensured that we sent the same probiotic from our previous investigation for thorough testing.
Thus, we dispatched the Seed DS-01 to an EPA-certified laboratory to evaluate contaminants. We specifically tested for four heavy metals: lead, cadmium, arsenic, and mercury. Additionally, we sought indications of PFAS “forever chemicals,” glyphosate, AMPA, and phthalates. The findings were very favorable.
Lab Results of Seed Daily Synbiotic DS-01
Below are the comprehensive results from the lab, along with some contextual information.
- PFAS “Forever Chemicals”: Non-detect organic fluorine
- 40 PFAS “Forever Chemical” Assays: Non-detect
- Glyphosate: Non-detect
- AMPA (Aminomethylphosphonic Acid): Non-detect (a breakdown chemical of glyphosate)
- 300+ Additional Pesticides: Non-detect
- Phthalates: 242 ppb total phthalate (This is lower than the lowest in women’s multivitamins)
- Lead: 0.01 mcg/serving (No Prop. 65 warning needed)
- Cadmium: 0.01 mcg/serving (No Prop. 65 warning needed)
- Arsenic: 0.03 mcg/serving (No Prop. 65 warning needed)
- Mercury: Non-detect
Seed DS-01 has emerged as one of the cleanest supplements that Mamavation has independently tested. According to laboratory results, no glyphosate, AMPA, over 300 additional pesticides, specific PFAS “forever chemicals,” or organic fluorine (indicators of PFAS) were found.
A note on phthalates: Mamavation has detected phthalates in nearly every supplement we’ve analyzed, including those pending publication. We have a good understanding of what constitutes lower phthalate levels in the industry currently. The levels found in Seed DS-01 were indeed low, even lower than those found in the best women’s multivitamins, placing it in our “best” category. However, it’s worth mentioning that the only instances of non-detect phthalate results have been in some “gummies” and “chews” of children’s multivitamins; we have not encountered this with solid supplements yet. This calls for a closer examination, and we appreciate consumers bringing issues like this to light.
The four primary heavy metals examined—lead, cadmium, arsenic, and mercury—showed concentrations well below Prop. 65 warning thresholds. Hence, according to California’s strict regulations, Seed DS-01 does not require any warnings regarding heavy metals.
Special Offer to Mamavation Community From Seed
In celebration, Seed is offering a special discount to the Mamavation community. Enjoy $30 off your first month of Seed when you use this link; offer valid until March 31st, after which it will revert to $20.

More About Seed DS-01 & Bacterial Strains Clinically Tested
The DS-01 is a daily probiotic and prebiotic clinically proven to alleviate bloating, gas, and irregularity, while also providing long-term advantages for immune, skin, and heart health. DS-01 contains 24 bacterial strains, amounting to 53.6 billion AFU, which are rigorously tested for quality, pesticides, and allergens by Seed.
Only about 16% of supplements undergo clinical testing. The DS-01 has been clinically validated in four gold-standard, double-blind placebo-controlled studies. Moreover, its probiotic strains have been confirmed through ten dose-matched gold-standard clinical trials.
These specific bacterial strains, when used in combination, have been shown in clinical studies to significantly benefit gut, immune, skin, and heart health.
DS-01 Bacterial Strains
Here are the 24 bacterial strains & species found in DS-01:
Bifidobacterium longum SD-BB536-JP, Bifidobacterium breve SD-BR3-IT, Lactobacillus plantarum SD-LP1-IT, Lactobacillus rhamnosus SD-LR6-IT, Lactobacillus reuteri SD-LRE2-IT, Bifidobacterium longum HRVD90b-US, Bifidobacterium lactis RD-BS5-IT, Lactobacillus casei HRVD300-US, Bifidobacterium breve HRVD521-US, Bifidobacterium lactis HRVD524-US, Lactobacillus rhamnosus HRVD113-US, Bifidobacterium lactis SD-150-BE, Lactobacillus rhamnosus SD-GG-BE, Lactobacillus reuteri SD-RD830-FR, Bifidobacterium adolescentis SD-BA5-IT, Lactobacillus crispatus SD-LCR01-IT, Lactobacillus fermentum SD-LF8-IT, Lactobacillus plantarum SD-LPLDL-UK, Bifidobacterium lactis SD-MB2409-IT, Lactobacillus salivarius SD-LS1-IT, Bifidobacterium lactis SD-CECT8145-SP, Bifidobacterium longum SD-CECT7347-SP, Lactobacillus casei SD-CECT9104-SP.
Digestive Health
DS-01 has been formulated to enhance bowel movement frequency and improve stool consistency for a more comfortable experience.
Clinical Trial Results
Immune Health
DS-01 is designed to support a strong and resilient immune system. The designated strains enhance the gut-immune connection, allowing for effective communication between systems and bolstering overall immune health.
Clinical Trial Results
Skin Health
DS-01 is composed of targeted strains that support the gut-skin axis, a critical element in controlling inflammatory pathways between the gut and skin.
Clinical Trial Results
Heart Health
DS-01 is formulated to assist in maintaining healthy, stable levels of cholesterol and blood pressure. Select strains stimulate the production of beneficial metabolites in the gut, thereby supporting healthy liver function and cholesterol management.
Clinical Trial Results
Gut Barrier
DS-01 is designed to fortify and support a robust gut barrier. The selected strains help replenish gut microflora, aiding in the restoration of a weakened microbiome.
Clinical Trial Results
Special Offer to Mamavation Community From Seed
In celebration, Seed is offering a discount exclusively for the Mamavation community. Enjoy $30 off your first month of Seed by using this link.
